MOESM1 of Microbial biosynthesis of lactate esters

Additional file 1: Table S1. Summary of high cell density cultures of EcJW101, EcJW102, EcJW103, EcJW104, and EcJW105 with addition of glucose and various alcohols after 24 h. The subscripts i and f are referred to the initial (0 h) and final (24 h) time of the culture, respectively. Table S2. Summary of titer, specific productivity, and yield of esters in high cell density cultures of EcJW101, EcJW102, EcJW103, EcJW104, and EcJW105 with addition of glucose and various alcohols after 24 h. The acyl acetate and acyl lactate columns correspond to the acyl alcohols added. For example, with the exogenous addition of ethanol, the acyl acetate, and acyl lactate columns represent ethyl acetate, and ethyl lactate, respectively. Table S3. (A) Summary of high cell density cultures of EcDL002, EcJW201, and EcJW202 after 24 h. (B) Summary of bioreactor batch fermentation of EcJW201 after 18 h. The subscripts i and f are referred to the initial (0 h) and final (24 h) time of the culture, respectively. Table S4. Summary of high cell density cultures of EcJW106-108 and EcJW203-208 with different concentrations of IPTG (0.01, 0.1, or 1.0 mM) after 24 h. The subscripts i and f are referred to the initial (0 h) and final (24 h) time of the culture, respectively. Table S5. Summary of titer, specific productivity, and yield of esters in high cell density cultures of EcJW106-108 and EcJW203-208 with different concentrations of IPTG (0.01, 0.1, or 1.0 mM) after 24 h. Table S6. Summary of high cell density cultures of EcJW209-212 with or without addition of ethanol (2 or 10 g/L) after 24 h. (A) OD600, pH, glucose, lactate, and ethanol. (B) Titer, specific productivity, and yield of esters. The subscripts i and f are referred to the initial (0 h) and final (24 h) time of the culture, respectively. Table S7. Summary of high cell density cultures of EcJW213-221 after 24 h. (A) OD600, pH, glucose, lactate, and ethanol. (B) Titer, specific productivity, and yield of esters. The subscripts i and f are referred to the initial (0 h) and final (24 h) time of the culture, respectively. Table S8. Summary of high cell density cultures of EcJW109-117 after 24 h. (A) OD600, pH, glucose, lactate, and ethanol. (B) Titer, specific productivity, and yield of esters. The subscripts i and f are referred to the initial (0 h) and final (24 h) time of the culture, respectively.

附加文件1：表S1 为EcJW101、EcJW102、EcJW103、EcJW104及EcJW105在添加葡萄糖与多种醇类后，培养24小时的高密度细胞培养总结。下标i与f分别对应培养初始时刻（0 h）与终末时刻（24 h）。表S2 为EcJW101、EcJW102、EcJW103、EcJW104及EcJW105在添加葡萄糖与多种醇类后，培养24小时的高密度细胞培养中酯类的效价、比生产率与得率总结。乙酸酯列与乳酸酯列与所添加的醇类相对应。例如，外源添加乙醇时，乙酸酯列与乳酸酯列分别代表乙酸乙酯与乳酸乙酯。表S3 (A) 为EcDL002、EcJW201及EcJW202培养24小时后的高密度细胞培养总结；(B) 为EcJW201生物反应器分批发酵18小时后的总结。下标i与f分别对应培养初始时刻（0 h）与终末时刻（24 h）。表S4 为EcJW106-108与EcJW203-208在不同浓度异丙基-β-D-硫代半乳糖苷（IPTG，0.01、0.1或1.0 mM）处理下，培养24小时后的高密度细胞培养总结。下标i与f分别对应培养初始时刻（0 h）与终末时刻（24 h）。表S5 为EcJW106-108与EcJW203-208在不同浓度IPTG（0.01、0.1或1.0 mM）处理下，培养24小时的高密度细胞培养中酯类的效价、比生产率与得率总结。表S6 为EcJW209-212在添加或不添加乙醇（2或10 g/L）条件下，培养24小时后的高密度细胞培养总结。(A) 包含OD600、pH、葡萄糖、乳酸与乙醇含量；(B) 包含酯类的效价、比生产率与得率。下标i与f分别对应培养初始时刻（0 h）与终末时刻（24 h）。表S7 为EcJW213-221培养24小时后的高密度细胞培养总结。(A) 包含OD600、pH、葡萄糖、乳酸与乙醇含量；(B) 包含酯类的效价、比生产率与得率。下标i与f分别对应培养初始时刻（0 h）与终末时刻（24 h）。表S8 为EcJW109-117培养24小时后的高密度细胞培养总结。(A) 包含OD600、pH、葡萄糖、乳酸与乙醇含量；(B) 包含酯类的效价、比生产率与得率。下标i与f分别对应培养初始时刻（0 h）与终末时刻（24 h）。