Transport stress induces paradoxical increases in airway inflammatory responses in beef stocker cattle

The development of Bovine Respiratory Disease (BRD ) in beef cattle is associated with stressful events, including auction and transport. To characterize immunity at the primary site of pathogen colonization in BRD, we evaluated stress responses in lung cells from beef stocker cattle to understand mucosal immune changes. Stocker calves were sampled via bronchoalveolar lavage fluid collection within 24 hours of auction and transport to a new facility (Stressed, n=12), or allowed to acclimate for 2 months at the new facility before sampling (Acclimated, n=7). Lavage cellular RNA was extracted and sequenced for bulk RNA-seq gene expression. The resulting fastq files are included in this repository and named based on the calf's unique identifier at sampling. The metadata file provides the key to which experimental group each calf belongs, as well as pertinent sequencing metadata values and MD5 sums to ensure file quality. Raw data counts are included in the counts.txt file, and are org..., , # Transport stress induces paradoxical increases in airway inflammatory responses in beef stocker cattle

Dataset DOI: [10.5061/dryad.02v6wwqh6](https://doi.org/10.5061/dryad.02v6wwqh6)

## Description of the data and file structure

This study was conducted to evaluated differences in gene expression from bronchoalveolar lavage fluid cells between Stressed calves (collected within 24 hours of arrival at a new facility, n=12) and Acclimated calves that experienced the same stress, but were allowed to acclimated for 2 months to the facility before sampling (n=7). All cattle were Bos Taurus, Angus cross cattle and were sampled between 6-8 months of age. After sampling cattle via aseptic technique, samples were transported on ice to the processing lab, where they were washed with 1X phosphate-buffered saline (PBS), filtered through a sterile 70 micron filter, and pelleted via centrifugation at 500g for 10 minutes. Supernatant was removed and cells were lysed using RLT lysis buffer from Qia...,