one-to-one orthologs

Manakins represent a family of small suboscine passerine birds characterized by their elaborate courtship displays, non-monogamous mating system, and sexual dimorphism. This family thus serves as a good model for the study of sexual selection. Here we present the genome assemblies of four manakin species (<em>Cryptopipo holochlora, Dixiphia pipra, Machaeropterus deliciosus</em> and <em>Masius chrysopterus</em>) generated by Single-tube Long Fragment Read (stLFR) technology. The assembled genome sizes ranged from 1.10 Gb to 1.19 Gb, with average scaffold N50 of 28 Mb and contig N50 of 169 Kb. On average, 9.82% genome region was annotated as repetitive elements, and 12,059 protein-coding genes were annotated in the genomes. We further identified about 75 Mb Z-linked sequences in manakins, containing 585 to 751 genes and an ~600 Kb pseudoautosomal region (PAR). One interesting finding of these Z-linked sequences is that a possible Z-to-autosome/PAR reversal could happen in <em>Masius chrysopterus</em>. These manakin genomes will contribute to a deeper understanding of evolutionary history and sexual selection in these species.

娇鹟科（Manakins）是一类小型亚鸣禽雀形目鸟类，以其复杂精巧的求偶炫耀行为、非一夫一妻制的交配系统以及性二态性为典型特征，因此该类群是开展性选择研究的理想模型。本研究报道了4种娇鹟的基因组组装结果，涉及物种分别为*Cryptopipo holochlora*、*Dixiphia pipra*、*Machaeropterus deliciosus*以及*Masius chrysopterus*，所有组装均采用单管长片段读取（Single-tube Long Fragment Read, stLFR）技术完成。组装后的基因组大小介于1.10 Gb至1.19 Gb之间，平均支架N50（scaffold N50）为28 Mb，平均重叠群N50（contig N50）为169 Kb。基因组注释结果显示，平均9.82%的基因组区域被注释为重复序列元件，每个基因组平均注释得到12059个蛋白质编码基因。本研究进一步在娇鹟基因组中鉴定出约75 Mb的Z染色体连锁序列，该区域包含585至751个基因，以及一段长度约600 Kb的假常染色体区域（pseudoautosomal region, PAR）。针对上述Z染色体连锁序列的一项有趣发现为：*Masius chrysopterus*中可能存在Z染色体-常染色体/假常染色体区域的反转事件。本研究获得的娇鹟基因组数据将为深入解析该类群的演化历史与性选择机制提供重要支撑。