Identifying hand2 downstream targets in cardiomyocyte during early cardiogenesis

hand2 reporter-expressing cardiac cells fail to migrate to the midline. To investigate the underlying molecular changes, we sequenced mRNA from 3900 and 3836 hand2 reporter-expressing single cells from 24 hpf hand2 FLD-/- and hand2 FLD+/? sibling embryos, respectively. Furthermore, hand2 is broadly expressed within the LPM, which gives rise to various cell lineages and tissues including the cardiovascular system, blood, kidneys, mesothelium, and limb connective tissue. To further investigate the requirement of Hand2 in the early events of cardiac lineage specification in zebrafish, we aim to characterize the early hand2 expressing cardiac precursors by using embryonic CM H3K27ac ChIP-seq and ATAC-seq data. Overall design: scRNA-seq: WT: hand2 reporter-expressing single cells from 24 hpf hand2 FLD+/? Embryos; mutants: hand2 reporter-expressing single cells from 24 hpf hand2 FLD-/- sibling embryos. ATAC-seq and CGIP-seq: Comparing myl7:GFP+ CMs with myl7:GFP- cells