SAIMOS - Biological and Flow Cytometry data collected from sampling stations in South Australia, in February 2020

Flow cytometry data was collected in February 2020, in waters off South Australia, from the Gulf St Vincent (SAMGSV).

The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.

Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

Since July 2019 two additional BGC stations have been added to the SAIMOS sampling array: the SA Upper Spencer Gulf Mooring (SAMUSG, 33.111S, 137.709E) and the SA Gulf St Vincent Mooring (SAMGSV, 35.023S, 138.46E).

2020年2月，于澳大利亚南部海域圣文森特湾（Gulf St Vincent, SAMGSV）采集流式细胞术（flow cytometry）数据。
本研究的核心目标为：建立澳大利亚南部大陆架海域生态系统的基础背景资料，明确上升流/盐度流出事件对微生物群落（microbial communities）的影响，最终构建该区域的生物地球化学模型。采样工作由"RV Ngerin"号科考船执行，隶属于澳大利亚南部综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目。每次科考航次均对叶绿素荧光最大值层（chlorophyll fluorescence maximum, DCM）的物理、化学及生物学特性开展调查。本次研究采集的流式细胞术数据涵盖超微型浮游植物（picophytoplankton）、细菌与病毒。
本研究共设置6个主要采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）及SAM5CB/B5（35.008°S，135.198°E）；剩余1个为位于袋鼠岛西南侧的外海站位B1（36.188°S，136.178°E）。需注意的是，结合站位间距（14~25海里）、陆架中层海流的平均流速分量（0.01 m·s⁻¹）以及科考船的平均航速（9节），可判定各站位所采集的水体团各不相同。
研究期间还会偶尔从袋鼠岛国家参考站位（National Reference Station, NRS，35.832°S，136.447°E）以及发生盐度流出事件的南澳斯宾塞湾口锚站位（SA Spencer Gulf Mouth Mooring, SAM8SG，35.25°S，136.690°E）采集补充样本。
自2019年7月起，SAIMOS采样阵列新增2个生物地球化学（Biogeochemical, BGC）观测站位：南澳斯宾塞湾上部锚站位（SA Upper Spencer Gulf Mooring, SAMUSG，33.111°S，137.709°E）与南澳圣文森特湾锚站位（SA Gulf St Vincent Mooring, SAMGSV，35.023°S，138.46°E）。