SH1036294.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的解决方案，用于界定、识别、传播及处理基于DNA的物种假说（Species Hypotheses，简称SH）。国际核苷酸序列数据库中的全部真菌ITS（Internal Transcribed Spacer）序列，均通过应用一组动态距离阈值（0.5%~3.0%）聚类至近似物种水平。所有物种假说均会被赋予唯一且稳定的数字对象标识符（Digital Object Identifier，DOI）格式名称，其分类学与生态学注释信息将通过分布式网络第三方注释工作完成验证。所有SH均会被关联至对应分类单元名称及其尽可能明确的分类层级（如门、纲、目等），该关联过程将综合该SH下所有序列的鉴定结果完成。研究人员会为每个此类SH挑选一条经自动或人工指定的代表序列。这些序列已通过https://unite.ut.ee/repository.php 对外发布，供科研社区用于本地序列相似性搜索、下一代测序分析流程等研究场景。随着公共真菌ITS序列数量的持续增长，该系统及其数据集将自动完成更新。