Appendix 4.3.4: MEME selected site analysis

After identification of the selected branch via the aBSREL method, we turned to the MEME method within the HyPhy program (v2.3.11)[235], to identify those sites which may have adaptively evolved. We tested the branch leading to EAncLuc, which was identified as under selection in the aBSREL analysis. A single partition was recovered with 25 sites under episodic diversifying positive selection at p &lt;= 0.1.<br><br>

我们通过自适应分支位点随机效应似然（aBSREL）方法完成选定分支的鉴定后，转而使用HyPhy软件（v2.3.11）中的混合效应演化模型（MEME）方法[235]，以识别可能发生适应性演化的位点。本次分析针对经aBSREL分析鉴定为处于选择压力下的、指向EAncLuc的分支展开。

最终获得一个序列分区，其中包含25个在p值≤0.1的显著性水平下发生间歇性多样化正选择的位点。