Expression data from roots of WT and bts-3 plants exposed to either Fe sufficient or Fe deficient conditions for 72 hours

We wanted to understand at what level BTS acts, i.e. how upstream BTS acts and if BTS misregulation affets only a subset or multiple subsets of Fe regulated genes. We studied WT and bts-3 mutant roots. Using microarray analysis, we demonstrated that bts-3 has a consitutitive expression of Fe deficiency response in root. RNA was harvested from root tissues from WT and bts-3 plants grown on B5 for 2 weeks and then transferred to either +Fe or -Fe medium for 3 days. Two biological replicates per condition were used for WT and 3 biological replicates were used for bts-3.