Transcriptional Profiling of NASH mouse models

We performed RNA-seq of livers obtained from 2 different NASH mouse models NASH was induced in mice using two different approaches. In the first study, male C57BL/6 mice were fed with a methionine and choline deficient diet (Reseach Diet (RD): A02082002B) or a matched control low-fat diet (CD) (RD: A02082003B) for 4 weeks whereafter livers were collected for RNA-seq analyses. In a second study, male C57BL/6 mice were treated with streptozotocin at postnatal day 2 and fed with HFD from week 4 and onwards as previously described (STAM model, Fujii et al., 2013). Livers were collected at 4 weeks and 10 weeks of age followed by RNA-seq analyses.