Spatial multi-omics reveals vulnerabilities of radial glia-like cancer-initiating cells within distinct ecosystems of pediatric and adult malignant gliomas

We collected five DMG, five GBM (including two secondary GBM), and one peritumor samples from eleven patients requiring surgical resection. To establish an atlas for spatially-resolved gene expression, we performed short-read spatial transcriptomic sequencing on the tissue sections using the 10x Visium platform. Here, we established a spatial atlas of H3K27M-DMG and GBM through short read spatial transcriptomics to reveal the niche-specific distribution and vulnerability of stem-like cells in H3K27M-DMG and GBM