Neutralization of acidosis reduced CHOP expression and apoptosis in HR-treated HUVECs.
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The pH of culture media was adjusted to 7.4 with NaOH (1N) immediately after hypoxia exposure, followed by 6 h of reoxygenation. Then both cells and supernatants were harvested. (A) The expressions of cleaved caspase-3 and CHOP were determined by immunoblotting assay. (B–C) Quantitative analyses of the relative levels of protein expression. Cleaved caspase-3 and CHOP protein bands were quantified and normalized to β-actin. The mean value obtained from the control was arbitrarily defined as 1. (D) The percentage of LDH release in the supernatant. Data were expressed as mean ± SD from 4–6 separate experiments. *P
创建时间：
2016-02-23
