RNA-seq of homogenized lungs of APOE knock-in mice during COVID-19 I

Here we analyze the transcriptional profiles of homogenized lungs resected from APOE2, APOE3, and APOE4 knock-in mice in the absence of infection (day 0) and on days 2 and 4 post infection with SARS-CoV-2 MA10. Weighted gene co-expression analysis identified gene modules enriched for genes implicated in T and B cell activation to be downregulated in APOE2 and APOE4 mice during COVID-19 progression. Weighted gene co-expression network analysis and pathway analysis of RNA-sequencing data.本研究对APOE2、APOE3及APOE4基因敲入小鼠的匀浆化切除肺组织进行转录组谱分析，样本采集时点包括未感染状态（第0天），以及感染SARS-CoV-2 MA10毒株后的第2天与第4天。通过加权基因共表达分析（Weighted gene co-expression analysis）发现，在新冠疾病进展过程中，APOE2与APOE4小鼠体内富集了涉及T、B细胞活化相关基因的基因模块呈现下调表达。本研究基于RNA测序（RNA-sequencing）数据，完成了加权基因共表达网络分析与通路分析。