Transmission of Arsenophonus from single infected females vs mixed infected/uninfected groups

Two treatments: a) Single <em>Pachycrepoideus vindemmiae</em> females infected with <em>Arsenophonus</em> were allowed to oviposit on <em>D.melanogaster </em>pupae within a vial. F1 progeny were then tested for Arsenophonus presence via PCR (with QC on insect to verify amplifiability). b) Groups of 2 infected and 2 uninfected P. vindemmiae females were allowed to oviposit on <em>D.melanogaster </em>pupae within a vial. F1 progeny were then tested for <em>Arsenophonus</em> presence via PCR (with QC on insect to verify amplifiability). <br> Column A: Sample # Colimn B: Treatment (single female or group lay as defined above) Column C: Vial replicate number Column D: QC PCR results on insect DNA (1 = amplifiable 0= fail) Column E: PCR assay results for Arsenophonus presence (1 = present, 0 = absent) Column F: PCR plate/well Column G: Notes, e.g. if sample was rerrun when first assay was ambiguous.

两种实验处理设置如下：
a) 取单头感染*Arsenophonus*的*Pachycrepoideus vindemmiae*雌蜂，使其在试管内的*Drosophila melanogaster*（缩写为D.melanogaster）蛹上产卵。随后通过PCR技术（以昆虫样本质量控制验证扩增有效性）检测其子一代（F1）是否携带*Arsenophonus*。
b) 每组置入2头感染*Arsenophonus*与2头未感染*Arsenophonus*的*Pachycrepoideus vindemmiae*雌蜂，使其在试管内的*Drosophila melanogaster*蛹上产卵。随后通过PCR技术（以昆虫样本质量控制验证扩增有效性）检测其子一代（F1）是否携带*Arsenophonus*。

各列说明如下：
列A：样本编号
列B：实验处理类型（即上述单头雌蜂产卵或群组产卵模式）
列C：试管重复编号
列D：昆虫DNA的PCR质量控制结果（1表示可扩增，0表示扩增失败）
列E：*Arsenophonus*存在性PCR检测结果（1表示目标菌存在，0表示目标菌不存在）
列F：PCR反应板/孔位信息
列G：备注信息，例如首次检测结果模棱两可时的重测样本。