Amplicon sequencing of liver tissues to detect barcodes in multiplexed AAV genomes

14 different AAV vectors were packaged with 14 barcoded AAV transgenes. NGS was used to de-multiplex the composition of the barcoded mix at the vector level, at the entry level (DNA) and at the expression level (cDNA) of the targeted tissues, which were human liver ex plants, murine liver, xenografted murine liver and non-human primate liver.