Genome-wide profile of RBPJ and differential H3K27ac profile upon treatment with HDAC-inhibitor apicidin.

The Notch signaling pathway regulates several differentiation and developmental processes in both pre- and post-natal life and its aberrant regulation leads to diseases, including cancer. Here, we investigated the genome-wide distribution of the transcription factor RBPJ and the differential regulation of H3K27ac upon pharmacological inhibition of histone deacetylase 3 (HDAC3) with apicidin in mouse pre-T (Beko) cells. Mouse pre-T cells (Beko) were analyzed by ChIP-Seq using an RBPJ-specific antibody. Subsequently, Beko cells were treated with gamma-secretase inhibitor (GSI) DAPT, HDAC3 inhibitor apicidin or DMSO as control and H3K27ac was analyzed by ChIP-Seq. Two replicates of each sample are included with the only exception of the input controls.