Identification and experimental validation of mitochondrial metabolism-related biomarkers in chronic rhinosinusitis with nasal polyps

In this paper, we show that mitochondrial metabolism-related genes (MM-RGs) play a critical role in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). This is significant because, by integrating transcriptomic and single-cell RNA sequencing data, we identified five key biomarkers—CYP2J2, CYBB, ALOX5, PLB1, and PLA2G2A—which are enriched in immune-related pathways and strongly associated with M2 macrophage and plasma cell infiltration. Furthermore, our predictive nomogram and identification of epithelial and T cells as primary mediators, alongside validation through RT-qPCR, highlight novel diagnostic and therapeutic avenues for CRSwNP.

本研究证实，线粒体代谢相关基因（mitochondrial metabolism-related genes）在伴鼻息肉的慢性鼻-鼻窦炎（chronic rhinosinusitis with nasal polyps, CRSwNP）的发病机制中发挥关键作用。本研究通过整合转录组学与单细胞RNA测序（single-cell RNA sequencing）数据，鉴定出CYP2J2、CYBB、ALOX5、PLB1及PLA2G2A这5种关键生物标志物，上述标志物显著富集于免疫相关通路，且与M2型巨噬细胞及浆细胞浸润密切相关。此外，本研究构建的预测列线图、明确上皮细胞与T细胞为主要介导细胞，以及通过实时定量聚合酶链反应（RT-qPCR）开展的验证工作，为伴鼻息肉的慢性鼻-鼻窦炎提供了全新的诊断与治疗思路。