Selective modulation of gene expression in activated normal human peripheral blood mononuclear cells by store-operated calcium entry blocker BTP2

Calcium is a critical signaling molecule in many cell types including immune cells. The calcium-release activated calcium channels (CRAC) responsible for store-operated calcium entry (SOCE) in immune cells are gated by STIM family members functioning as sensors of Ca2+ store content in the endoplasmic reticulum. We investigated the effect of SOCE blockers BTP2 and CM4620 on human peripheral blood mononuclear cells (PBMCs) polyclonally-stimulated with the mitogen phytohemagglutinin (PHA). To investigate the effect of SOCE blocker BTP2 on human peripheral blood mononuclear cells (PBMCs) stimulated with the mitogen phytohemagglutinin (PHA), we performed bulk RNA sequencing (RNA-seq) and multiplexed single-cell RNA sequencing to query gene expression at the whole transcriptome level in human PBMCs from four healthy donors.