The Hubbard Brook Stream Ecology Record: Algal Biomass (Chlorophyll-a), 2018 - ongoing

The Hubbard Brook Stream Ecology record is a companion dataset to the Hubbard Brook Watershed Stream and Precipitation Chemistry record. The Stream Ecology record started in 2018 and HBWatER collects ecological samples from seven gauged watersheds: Watersheds 1 through 6 and Watershed 9. HBWatER measures algal biomass, aquatic invertebrate emergence, and stream decomposition by measuring (1) chlorophyll-a on tiles and artificial moss, which approximate algal biomass growth on bare rock and bryophyte mats, (2) preserved algal biomass on artificial moss substrates in Lugol’s Iodine solution, (3) aquatic invertebrate emergence on replicate sticky traps placed above the stream, and (4) stream decomposition through leaf litter pack and cotton strip decay. To complement these ecological records, HBWaTER installed light sensors and field cameras to obtain better information about the light and stream environment daily. Three replicate light sensors that take sub-daily measurements of light level intensity are placed at each watershed at the weir pond (full-sun), and two under the canopy (partial shade). Field cameras take daily photos at noon of the stream canopy and the stream channel. While many studies at Hubbard Brook have measured algal biomass, aquatic invertebrates, and stream decomposition, they are scattered in locations across the valley, were performed at non-continuous times, and use various semi-comparable methods. The HBWatER Stream Ecology record was created to address this gap and systematically measure any long-term changes in the organisms living in the stream.

The collection of HBWatER samples is currently sustained by Tammy Wooster (Cary IES) and analyses of these samples has been performed by Heather Malcom (Cary IES), Audrey Thellman (Duke), Steve Anderson (Duke), Geoff Wilson (Cary IES), and Adam Rok (Duke). The dataset is curated and maintained by a team of researchers: Chris Solomon (Cary IES), Emma Rosi (Cary IES), and Emily Bernhardt (Duke). Current Financial Support for HBWatER is provided by NSF LTREB #1907683 and the NSF LTER #XXX (I think you mentioned that you know the LTER fund code for this).

Data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.

哈伯布鲁克溪流生态数据集（Hubbard Brook Stream Ecology Record）是哈伯布鲁克流域溪流与降水化学数据集的配套数据集。该数据集于2018年启动，HBWatER项目组从7个监测流域（1至6号流域及9号流域）采集生态样本。HBWatER的测定指标包括藻类生物量、水生无脊椎动物羽化情况及溪流分解速率，具体通过以下方式开展：(1) 对瓦片与人工苔藓上的叶绿素a（chlorophyll-a）进行测定，以此估算裸岩与苔藓垫上的藻类生物量生长情况；(2) 对卢戈氏碘液（Lugol’s Iodine Solution）中保存的人工苔藓基质上的藻类生物量进行检测；(3) 在溪流上方布设重复式粘性诱捕器，监测水生无脊椎动物的羽化情况；(4) 通过枯叶分解袋与棉条降解实验测定溪流分解速率。为完善上述生态观测数据，HBWatER还部署了光传感器与野外相机，以每日获取更全面的光照与溪流环境信息。每个流域的堰塘（全日照环境）处设置3台可开展亚日光照强度测量的重复光传感器，同时在林冠下方（半遮阴环境）布设2台。野外相机每日正午拍摄溪流林冠与河道的影像。过往哈伯布鲁克的多项研究虽已涉及藻类生物量、水生无脊椎动物与溪流分解速率的测定，但这些研究分布于山谷各处、观测时间不连续，且采用了多种半可比的实验方法。HBWatER溪流生态数据集的建立正是为了填补这一研究空白，系统监测溪流生物群落的长期动态变化。

目前，HBWatER的样本采集工作由塔米·伍斯特（Tammy Wooster，卡里生态系统研究所(Cary IES)）负责，样本分析工作由希瑟·马尔科姆（Heather Malcom，卡里生态系统研究所）、奥黛丽·瑟尔曼（Audrey Thellman，杜克大学）、史蒂夫·安德森（Steve Anderson，杜克大学）、杰夫·威尔逊（Geoff Wilson，卡里生态系统研究所）与亚当·罗克（Adam Rok，杜克大学）共同完成。本数据集由克里斯·所罗门（Chris Solomon，卡里生态系统研究所）、艾玛·罗西（Emma Rosi，卡里生态系统研究所）及埃米莉·伯恩哈特（Emily Bernhardt，杜克大学）组成的研究团队进行管理与维护。HBWatER当前的经费支持来自美国国家科学基金会长期生态生物学研究项目（NSF LTREB #1907683）以及美国国家科学基金会长期生态研究网络项目（NSF LTER #XXX，此处沿用原文标注）。

本数据集的数据采集工作作为哈伯布鲁克生态系统研究（Hubbard Brook Ecosystem Study, HBES）的一部分开展。哈伯布鲁克生态系统研究是设于哈伯布鲁克实验林的合作研究项目，该实验林由美国农业部林务局（USDA Forest Service）北方研究站运营维护。