Effect of 2-deoxyglucose on the interferon gamma-mediated response to intracellular bacterial infection in mouse bone marrow-derived macrophages

We report transcriptional responses of mouse macrophages to Pam3CSK4 stimulation or L. pneumophila infection that are additionally stimulated with interferon gamma ± 2-deoxyglucose. We identify a strong transcriptional signature of the unfolded protein stress response in macrophages exposed to 2-deoxyglucose. Additionally we find that 2-deoxyglucose reduces the expression of several key interferon gamma-induced transcripts associated with an antimicrobial response to L. pneumophila. Examination of RNA isolated from mouse bone marrow-derived macrophages stimulated with Pam3CSK4 ± interferon gamma ± 2-deoxyglucose or infected with L. pneumophila ± interferon gamma ± 2-deoxyglucose