Data for "<i>Wolbachia</i> strain <i>w</i>MelM disrupts egg retention<i> </i>by <i>Aedes aegypti</i> females prevented from ovipositing"

<i>Aedes aegypti</i> mosquitoes are adapted to dry climates and can retain their eggs for extended periods in the absence of suitable habitat. <i>Wolbachia </i>strains transferred to mosquitoes can be released to combat dengue transmission by blocking virus replication and spreading through populations, but host fitness costs imposed by <i>Wolbachia</i>, particularly under some environments, can impede spread. We assessed the impact of two <i>Wolbachia</i> strains being released for dengue control (<i>w</i>AlbB and <i>w</i>MelM) on fecundity and egg viability following extended egg retention (12 or 18 d) under laboratory conditions. Egg viability decreased to a greater extent in females carrying <i>w</i>MelM compared to uninfected or <i>w</i>AlbB females. Fertility fully recovered in uninfected females following a second blood meal after laying retained eggs, while <i>w</i>MelM females experienced only partial recovery. Effects of <i>w</i>MelM on egg retention were similar regardless of whether females were crossed to uninfected or <i>w</i>MelM males, suggesting that fitness costs were triggered by <i>Wolbachia</i> presence in females. The fertility of <i>w</i>MelM females declined with age, regardless of whether females used stored sperm or were recently inseminated. Costs of some <i>Wolbachia</i> strains during egg retention may affect the invasion and persistence of <i>Wolbachia</i> in release sites where larval habitats are intermittent.埃及伊蚊（Aedes aegypti）适应干旱气候，可在缺乏适宜滋生环境的条件下长期留存虫卵。沃尔巴克氏体（Wolbachia）菌株被转移至蚊体后，可通过阻断病毒复制并在种群中扩散以防控登革热传播，但沃尔巴克氏体对宿主施加的适合度成本，尤其是在部分环境条件下，会阻碍其种群扩散。本研究在实验室条件下，针对两种用于登革热防控的沃尔巴克氏体菌株（wAlbB与wMelM），评估了虫卵经12天或18天长期留存后，其对雌蚊繁殖力及卵存活率的影响。实验结果表明，与未感染雌蚊或携带wAlbB的雌蚊相比，携带wMelM的雌蚊卵存活率下降幅度更为显著。未感染雌蚊在产下留存虫卵后再次吸食血餐，其繁殖力可完全恢复；而携带wMelM的雌蚊仅能实现部分恢复。无论雌蚊与未感染雄蚊还是wMelM雄蚊交配，wMelM对虫卵留存的影响均无显著差异，这提示适合度成本由雌蚊体内的沃尔巴克氏体存在所触发。无论雌蚊是利用储存的精子还是近期完成受精，携带wMelM的雌蚊繁殖力均随日龄增长而下降。部分沃尔巴克氏体菌株在虫卵留存期间产生的适合度成本，可能会影响沃尔巴克氏体在幼虫栖息地间歇性出现的释放位点的入侵与持续定殖能力。