Retinal gene expression of low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice

Retinal gene expression was assessed from postnatal day (P) 8 old low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice with a Illumina mouse-WG6 expression BeadChip. RNA was extracted from the retinas of P8 Lrp5 knockout and wild type mice (n=3 each group). Total RNA (1ug each) were reverse transcribed, followed by a single in vitro transcription amplification to incorporate biotin-labeled nucleotide, and subsequent hybridization and staining with strepatavidin-Cy3 according to the manufacturer's instructions.本研究采用Illumina小鼠全基因组表达微珠芯片（Illumina mouse-WG6 expression BeadChip），对出生后第8天（P8）的低密度脂蛋白受体相关蛋白5（Lrp5）基因敲除小鼠的视网膜基因表达情况进行检测。实验从P8龄Lrp5基因敲除小鼠与野生型小鼠的视网膜中提取总RNA（每组n=3），将每份1μg的总RNA进行反转录，随后通过单次体外转录扩增掺入生物素标记核苷酸，再依照制造商说明书完成杂交及链霉亲和素-Cy3染色。