Reliability and repeatability of the 10H10C/6G6B and 10H10C/2C4B AlphaLISAs on quality control samples.
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The inter- and intra-assay variations of the two HtrA3 AlphaLISAs detecting QCs in IB and serum were determined using a HtrA3-L-S305A calibration curve in IB or analyte-depleted human serum. QCs representing high and medium concentrations of HtrA3 were created in bulk by spiking purified insect-expressed WT HtrA3-L protein in buffer and analyte-depleted human serum matrix respectively and frozen in aliquots. Results are from three independent experiments; two assays in triplicate and one assay in ten replicate wells. The intra-assay variation was calculated from one assay of ten replicate wells.
创建时间：
2013-02-19
