Safety assessment of Spirulina platensis through sprague dawley rats modeling

Abstract Health safety of spirulina (Spirulina platensis) for food application was evaluated through hematology and histopathology of liver, spleen and kidney in Sprague Dawley rats fed on three isoproteic diets maintained to 10% protein for forty five days. Three groups of rats were maintained as control diet casein (R-C); diet containing crude spirulina (R-S) and diet containing purified spirulina protein (R-SPI). Hematological profile of the rats group fed on spirulina diets showed no significant increase in white blood cells (WBCs), while there was decreasing trend in the levels of neutrophils (60.46 ± 2.60%) and lymphocytes (33.53 ± 1.06%) in rats that were fed on spirulina diet (R-S) than the other tested experimental diet group. Histopathological examination revealed normal architecture of the liver with no abnormality in kidneys and spleen exhibiting safety of spirulina in both forms to mitigate protein energy malnutrition widely prevalent in developing countries.

摘要 本研究针对食品应用中的螺旋藻（Spirulina platensis）健康安全性展开评估。实验以斯普拉格-道利大鼠为对象，将其分为三组，喂食三种蛋白含量均为10%的等蛋白日粮，连续干预45天，通过血液学检测与肝、脾、肾组织病理学检查完成安全性评价。其中对照组喂食酪蛋白日粮（R-C组），实验组分别喂食粗制螺旋藻日粮（R-S组）与纯化螺旋藻蛋白日粮（R-SPI组）。血液学检测结果显示，喂食螺旋藻日粮的大鼠组白细胞（white blood cells, WBCs）计数无显著升高，但与其他实验组相比，R-S组大鼠的中性粒细胞（60.46±2.60%）与淋巴细胞（33.53±1.06%）水平呈下降趋势。组织病理学检查结果显示，大鼠肝脏结构正常，肾脏与脾脏无异常病变，表明两种形式的螺旋藻均具备食用安全性，可用于改善发展中国家广泛流行的蛋白质能量营养不良状况。