mRNA expression profile in BMCMCs from C57BL/6J and CC027 mice stimulated with or without IgE-ligation.

Collaborative Cross (CC) mice are a reproducible yet genetically diverse genetic reference panel derived from eight founder strains. Here, we compared the gene expression profiles of mast cells (MCs) from C57BL/6J (a traditional inbred strain) and CC027 (a CC strain) mice. We generated bone-marrow-derived cultured MCs (BMCMCs) from C57BL/6J and CC027 mice. The cells were sensitized with anti-DNP IgE overnight, then were stimulated with or without DNP-HSA, followed by total RNAs extraction. RNA-seq library construction and sequencing was done by Novogene (https://en.novogene.com/). Overall design: BMCMCs from C57BL6J and CC027 mice were sensitized with anti-DNP-IgE and stimulated with or without DNP-HSA for 1 hour, followed by RNA extraction. Then, RNA-sequencing was performed.