marine sediment metagenome Targeted loci environmental

The microbiological objectives of IODP Expedition 347 Baltic Sea Paleoenvironmentfocused on 1) how the phylogenetic diversity of the deep biosphere in this intracontinental sea differs from that of deep-ocean communities, and 2) are microorganisms that presently live in the deep sediments remnants of limnic and marine populations, or does the modern sedimentary environment select for thecommunity. The Expedition took place September 16, 2013 November 1, 2013 on board the IODP Mission-Specific Platform Greatship Manisha. Cores were advanced for microbiological sampling at 4 locations: BSB-1, BSB-3, BSB-7, and BSB-9 (Figure 1) using Advanced Piston Coring (APC) and checked for contamination using perfluorcarbon tracers (PFTs).

国际大洋发现计划（International Ocean Discovery Program, IODP）第347航次“波罗的海古环境”的微生物学研究目标聚焦于两大核心方向：一是该陆内海深部生物圈的系统发育多样性与深海群落存在何种差异；二是当前栖息于深部沉积物中的微生物，究竟是淡水与海洋种群的遗留类群，还是现代沉积环境筛选形成的特有群落。该航次于2013年9月16日至11月1日在IODP专项作业平台“曼尼沙号”（Greatship Manisha）上开展。科考团队在BSB-1、BSB-3、BSB-7及BSB-9共4个站位（见图1）采用先进活塞取芯（Advanced Piston Coring, APC）技术获取岩芯以开展微生物学采样，并借助全氟化碳示踪剂（perfluorcarbon tracers, PFTs）检测取芯过程中的污染情况。