Supplementary Material for: Cyclooxygenase-2 Gene Polymorphisms –765G>C and –1195A>G and Mycosis Fungoides Risk

<b><i>Background:</i></b> Cyclooxygenase-2 (COX-2) is an inducible modulator of inflammation that acts through increasing prostaglandin levels and has been described as a major mediator linking inflammation to cancer. Previous studies supported that COX-2<i></i>–765G&gt;C and –1195A&gt;G polymorphisms were associated with increased risk of several solid tissue cancers as well as some hematological malignancies. <b><i>Objective:</i></b> The aim of the study was to elucidate the association between functional COX-2 genotypes (–765G&gt;C and –1195A&gt;G) polymorphisms and the risk of developing mycosis fungoides (MF). <b><i>Methods:</i></b> This was a hospital-based, case-control study of 70 MF patients and 100 MF-free controls. We genotyped COX-2 –1195A&gt;G, –765G&gt;C, and –8473T&gt;C polymorphisms by using the PCR-restriction fragment length polymorphism method. <b><i>Results:</i></b> The AA genotype in the COX-2 –1195A&gt;G gene polymorphism and the GC genotype in the COX-2 −765G&gt;C gene were significantly more frequent among MF patients compared to controls (<i>p</i>&lt; 0.001 and <i>p</i> = 0.002, respectively). <b><i>Conclusion:</i></b> The ­results indicate a possible role of COX-2 genes in the pathogenesis of MF. These novel findings may allow for notable future advances, as it will enable the identification of the ­individuals most susceptible to MF.

<b><i>背景：</i></b> 环氧合酶-2（Cyclooxygenase-2, COX-2）是一种可诱导性炎症调节因子，通过提高前列腺素水平发挥生物学功能，被视为连接炎症与癌症的核心介质。既往研究证实，COX-2的–765G>C与–1195A>G基因多态性与多种实体组织癌及部分血液系统恶性肿瘤的发病风险升高相关。<b><i>目的：</i></b> 本研究旨在阐明功能性COX-2基因多态性（–765G>C与–1195A>G）与蕈样肉芽肿（mycosis fungoides, MF）发病风险之间的关联。<b><i>方法：</i></b> 本研究为基于医院的病例对照研究，共纳入70例MF患者与100例无MF的对照人群。采用聚合酶链反应-限制性片段长度多态性（PCR-restriction fragment length polymorphism, PCR-RFLP）方法对COX-2基因的–1195A>G、–765G>C及–8473T>C多态性进行基因分型。<b><i>结果：</i></b> 与对照组相比，MF患者中COX-2 –1195A>G基因多态性的AA基因型以及COX-2 –765G>C基因的GC基因型检出率显著更高（分别为p<0.001和p=0.002）。<b><i>结论：</i></b> 本研究结果提示COX-2基因可能在蕈样肉芽肿的发病机制中发挥作用。这一新发现可为未来相关研究带来重要进展，助力精准识别MF高易感个体。