Data from: Efficiency of using electric toothbrush as an alternative to tuning fork for artificial buzz pollination is independent of instrument buzzing frequency

Background: Breeding programs and research activities where artificial
buzz pollinations are required have primarily relied upon using tuning
forks, and bumble bees. However, these methods can be expensive,
unreliable, and inefficient. To find an alternative, we tested the
efficiency of pollen collection using electric toothbrushes and compared
it with tuning forks at three vibration frequencies- low, medium, and high
and two extraction times at 3 seconds and 16 seconds- from two buzz
pollinated species (Solanum lycopersicum and Solanum elaeagnifolium).
Results: Our results show that species, and extraction time significantly
influenced pollen extraction, while there were no significant differences
for the different vibration frequencies and more importantly, the use of a
toothbrush over tuning fork. More pollen was extracted from Solanum
elaeagnifolium when compared to Solanum lycopersicum, and at longer
buzzing time of regardless of the instrument used. Conclusions: Our
results suggest that electric toothbrush can be a viable and inexpensive
alternative to tuning forks, and regardless of the instrument used and
buzzing frequency, time of buzzing is also critical in pollen extraction

背景：需开展人工振铃授粉（buzz pollination）的育种项目与研究活动，传统上主要依赖音叉与熊蜂。但此类方法存在成本高昂、效果不稳定且效率低下的问题。为寻找替代方案，我们以两种振铃授粉物种——普通番茄（*Solanum lycopersicum*）和银叶茄（*Solanum elaeagnifolium*）为研究材料，测试了电动牙刷收集花粉的效率，并将其与音叉在三种振动频率（低、中、高）及两种提取时长（3秒、16秒）下的花粉提取效果进行了对比。
结果：研究结果显示，植物物种与花粉提取时长对花粉提取效果具有显著影响；而不同振动频率未产生显著差异，更重要的是，使用电动牙刷相较于音叉也未出现显著统计学差异。相较于普通番茄，银叶茄的花粉提取量更高；且无论使用何种工具，更长的振铃时长均可提升花粉提取量。
结论：本研究结果表明，电动牙刷可作为音叉的一种经济可行的替代方案；且无论使用何种工具、振动频率如何，振铃时长均对花粉提取效果至关重要。