Figure 3: Gene Expression of MT and Ho-1

Figure 3: Gene expression analysis of mercury compounds in medaka embryos. Medaka embryos were sampled from control, 0.1 μM MeHg, 1 μM HgCl2, 10 μM α-HgS, and 10 μM β-HgS treatment groups (n=3, 15 embryos pooled per replicate) at 3 days post-exposure. Total RNA was extracted and subjected to RT-PCR analysis for metallothionein (<i>MT</i>) and heme oxygenase-1 (<i>Ho-1</i>) gene expression using β-actin expression as the reference. Data are mean ± SD. *Significantly different from controls with p &lt; 0.05.

图3：青鳉胚胎内汞化合物的基因表达分析。本实验设置对照组、0.1μM甲基汞（MeHg）、1μM氯化汞（HgCl₂）、10μM α-硫化汞（α-HgS）及10μM β-硫化汞（β-HgS）处理组，于暴露后3天采集胚胎样本（每组生物学重复数n=3，每个重复混合15枚胚胎）。提取总RNA后，以β-肌动蛋白（β-actin）的表达作为内参基因，通过逆转录聚合酶链反应（RT-PCR）检测金属硫蛋白（MT）与血红素加氧酶-1（Ho-1）的基因表达水平。实验数据以平均值±标准差（SD）呈现，*标记表示与对照组相比具有统计学显著性差异（p<0.05）。