Expression of Myostatin (Mstn) and Myogenin (Myog) Genes in Zi And Rhine Goose and Their Correlation with Carcass Traits

ABSTRACT The aim of this study was to investigate the effects of Myostatin (MSTN) and MyoGenin (MyoG) on goose skeletal muscle growth. In this study, MSTN and MyoG gene expression in breast and leg muscle of Zi and Rhine goose were detected by Real-time Polymerase Chain Reaction (PCR), and the correlations between genes expression levels and carcass traits were investigated. The results showed that the breast muscle weight and breast muscle percentage of Rhine goose were significantly higher than Zi goose (p<0.01). MSTN mRNA and MyoG mRNA expression in breast muscle of Zi goose were significantly higher than that of Rhine goose and the level of MSTN in leg muscle of Rhine was significantly higher than that of Zi goose (p<0.01). There was a significant difference between MSTN mRNA expression in breast muscle and in leg muscle of Zi goose (p<0.01). MSTN mRNA expression in leg muscle was significantly higher than that of breast muscle of Rhine goose (p<0.05). There was a significant difference between MyoG mRNA expression in breast muscle and in leg muscle of Zi goose and Rhine goose (p<0.01). There was a negative correlation between MSTN mRNA expression in breast muscle and body weight, breast muscle weight and breast muscle percentage.

摘要 本研究旨在探究肌抑素（Myostatin, MSTN）与肌生成素（MyoGenin, MyoG）对鹅骨骼肌生长的影响。本研究采用实时聚合酶链式反应（Real-time Polymerase Chain Reaction, PCR），检测了籽鹅（Zi goose）与莱茵鹅（Rhine goose）胸肌、腿肌中MSTN与MyoG基因的表达水平，并分析了基因表达量与屠宰性状之间的相关性。结果显示，莱茵鹅的胸肌重量与胸肌率均极显著高于籽鹅（p<0.01）。籽鹅胸肌中MSTN mRNA与MyoG mRNA的表达水平均极显著高于莱茵鹅，且莱茵鹅腿肌中MSTN的表达水平也极显著高于籽鹅（p<0.01）。籽鹅胸肌与腿肌中的MSTN mRNA表达水平存在极显著差异（p<0.01）。莱茵鹅腿肌中的MSTN mRNA表达水平显著高于其胸肌（p<0.05）。籽鹅与莱茵鹅的胸肌、腿肌中MyoG mRNA表达水平均存在极显著差异（p<0.01）。胸肌中MSTN mRNA的表达水平与体重、胸肌重量及胸肌率呈显著负相关。