Additional file 1 of Three-dimensional culture of MSCs produces exosomes with improved yield and enhanced therapeutic efficacy for cisplatin-induced acute kidney injury

Additional file 1: Supplementary Table 1. Primers used in this study. Figure S1. Identification of hucMSCs. a HucMSCs exhibited a spindle fibroblast-like morphology. Scale bar: 50 μm. b HucMSCs could differentiate into osteoblasts, adipocytes or chondroblasts, evidenced by Alizarin Red S staining (b-a), Oil Red O staining (b-b), and Alcian Blue/Nuclear Fast Red staining (b-c), respectively. Scale bar: 50 μm. c Flow cytometry analysis of MSC (CD29, CD44, CD73 and CD90) and HSC (CD34 and CD45) surface markers. Blue solid peaks represent the isotype controls and the red solid peaks represent the marker indicated.

附加文件1：补充表1。本研究使用的引物。
图S1：人脐带间充质干细胞（human umbilical cord mesenchymal stem cells，hucMSCs）的鉴定。
a hucMSCs呈纺锤状成纤维细胞样形态。比例尺：50 μm。
b hucMSCs可分化为成骨细胞、脂肪细胞及软骨细胞，分别通过茜素红S染色（b-a）、油红O染色（b-b）与阿尔辛蓝/核固红染色（b-c）得以证实。比例尺：50 μm。
c 流式细胞术分析间充质干细胞（mesenchymal stem cells，MSC）的表面标志物CD29、CD44、CD73与CD90，以及造血干细胞（hematopoietic stem cells，HSC）的表面标志物CD34与CD45：蓝色实心峰代表同型对照，红色实心峰代表所指示的标志物。