RUNX3 binds TEADs and YAP1

RUNX3 interacts with both YAP1 and TEAD proteins. The interaction with YAP1 involves the PY motif of RUNX3 and the WW domain of YAP1. The interaction with TEADs involves the Runt domain of RUNX3 and the DNA recognition helix of TEADs. RUNX3 was shown to directly interact with TEAD1 and TEAD4. Based on sequence similarity, it is highly probable that RUNX3 also interacts with TEAD2 and TEAD3. The interaction of RUNX3 with YAP1 and/or TEADs does not prevent formation of the YAP1:TEADs complex (Yagi et al. 1999, Qiao et al. 2015).

RUNX3蛋白与YAP1和TEAD蛋白均存在相互作用。与YAP1的相互作用涉及RUNX3的PY基序与YAP1的WW结构域。而与TEADs的相互作用则涉及RUNX3的Runt结构域与TEADs的DNA识别螺旋。研究表明，RUNX3可以直接与TEAD1和TEAD4蛋白相互作用。基于序列相似性分析，RUNX3与TEAD2和TEAD3蛋白的相互作用亦极有可能。值得注意的是，RUNX3与YAP1和/或TEADs的相互作用并不会阻碍YAP1:TEADs复合物的形成（Yagi et al. 1999, Qiao et al. 2015）。