RNA sequence data from krill CO2 exposure experiment

We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm).

These were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages * 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data).

本研究采用RNA测序（RNA sequencing）技术，探究二氧化碳浓度升高对南极磷虾体内遗传/生理通路的影响。研究团队于2012年3月在AAD水族馆开展南极磷虾幼体二氧化碳暴露实验，设置两个发育阶段（Calyptopis I期与Furcilia V期）以及三个二氧化碳浓度梯度（对照组、1000 ppm与2000 ppm）。
本次实验为短期暴露实验（时长2天）——因前期以受精卵为起始材料的长期暴露实验中，处理组与对照组间出现发育阶段差异，该差异会干扰实验数据的可靠性。研究人员从幼体样本中提取RNA，并对6个样本（2个发育阶段×3个处理组）开展高通量RNA测序（RNA-seq），测序工作在Illumina测序仪（Genome Analyzer II）上完成。每个样本约获得6000万条序列读段（数据以FASTA格式存储，每条读段对应100个碱基的序列信息），总计约3.6亿条读段，对应总数据量达360亿碱基对。