MOESM7 of Coincidence cloning recovery of Brucella melitensis RNA from goat tissues: advancing the in vivo analysis of pathogen gene expression in brucellosis

Additional file 7. Additional indel analysis. Data is presented from FreeBayes for a set of 5 selected indels identified by both FreeBayes and SNVer for coincidence cloning reads as compared to the B. melitensis 16M reference genome. For each of the samples indicated (provided as examples), the following are displayed: the total reads at the indel location from the RNA-sequencing sample, as mapped to the B. melitensis genome; the number of reads carrying the alternative (indel) allele; and the percent of mapped reads exhibiting the indel. >â 92% of reads in each displayed location carried the indel of interest.

附加文件7：补充插入缺失（insertion-deletion, indel）分析。本数据由FreeBayes软件生成，针对5个经FreeBayes与SNVer共同鉴定的插入缺失位点，基于共克隆读段与羊布鲁氏菌（B. melitensis）16M参考基因组完成比对分析。对于本次展示的示例样本，将呈现以下内容：该RNA测序样本中，比对至羊布鲁氏菌基因组的插入缺失位点处的总读段数；携带插入缺失型变异等位基因的读段数；以及呈现该插入缺失的比对读段占比。每个展示位点中，超过92%的读段携带目标插入缺失。