FGF2 regulation of gene expression in stable inducible Neurons

Stable inducible neurons were generated using Tol2 recombinase to generate human embryonic stem cells that expressed human NEUROG2 in a doxycycline regulated maner. Treatment with doxycycline caused neuronal differentiation of the stem cells (samples 55649-55653). The post-mitotic excitatory neurons derived from the stem cells were used to examine FGF2 regulation of gene expression (samples 57089-57096). Forced overexpression of human NEURGO2 was used to differentiate human embryonic stem cells (H9) into a homogeneous population of post-mitotic excitator neurons