Variation of proteomic profile during lactation in Girgentana goat milk: a preliminary study

The knowledge of milk proteome has been greatly enhanced by technological advances in the proteomics field as the use of the two-dimensional differential in-gel electrophoresis, a gel-based approach which allowed the analysis of proteins from complex mixtures and the comparing of several protein samples in the same experiment. The aim of this study was to characterise the whole milk proteomic profile in Girgentana dairy goat breed by two-dimensional differential in-gel elecrophoresis. The obtained representative 2D whole milk proteomic map showed a general picture of the protein distributions over the pH 3–10 NL including about 100 spots, most of them organised like a spot train. Among differentially abundant spots in the three experimental conditions, milk fat globule EGF factor 8 protein, β-lactoglobulin, β-casein and serum albumin were successfully identified. The three-dyes system employed in two-dimensional differential in-gel electrophoresis (2D-DIGE) analysis allowed us to obtain a global representation of the Girgentana whole milk proteome. These preliminary results could be used to generate a milk reference proteomics map for the Girgentana goat breed.

随着蛋白质组学领域技术的进步，尤其是二维差异凝胶电泳（two-dimensional differential in-gel electrophoresis，简称2D-DIGE）这一基于凝胶的研究方法的应用，人们对牛乳蛋白质组（milk proteome）的认知得到了极大提升；该方法可实现复杂混合物中蛋白质的分析，且能在同一实验中对多份蛋白质样品进行比较。
本研究旨在通过二维差异凝胶电泳，解析吉尔真塔（Girgentana）奶山羊品种的全乳蛋白质组谱。所获得的代表性二维全乳蛋白质组图谱，呈现了pH 3-10非线性范围内的蛋白质分布概况，共包含约100个蛋白点，其中多数呈点列状排布。在三种实验条件下存在丰度差异的蛋白点中，研究人员成功鉴定出乳脂肪球EGF因子8蛋白、β-乳球蛋白（β-lactoglobulin）、β-酪蛋白（β-casein）与血清白蛋白（serum albumin）。本研究在2D-DIGE分析中采用的三色标记系统，帮助我们获取了吉尔真塔奶山羊全乳蛋白质组的全局表征。本研究的初步结果可用于构建吉尔真塔奶山羊的牛乳参考蛋白质组图谱。