Chromatin accessibility profile of splenic LCMV-specific memory B cells following type I or II interferon blockade in acute versus chronic LCMV infection

Dynamics of interferon signalling impact the adaptive immune response. We used ATAC sequencing (ATAC-seq) to analyze chromatin accessibility in memory B cells from mice infected with acute or chronic LCMV infection, treated with type I or type II interferon blocking antibodies. Overall design: Splenic LCMV-specific memory B cells from LCMV-infected mice were isolated by fluorescence-activated cell sortings (FACS) and analysed using ATAC-seq.