IRS1 phosphorylation determines a heritable probability of cancer cells to persist during EGFR inhibition therapy [Mars-seq]

As part of a multi-omic pursuit for mechanisms of persistency of cancer cells to drug treatment, we generated pair-wise transcriptome analysis of nearly 200 single-cell-derived clonal populations, with and w/o drug treatment. Overall design: Single PC-9 cancer cells were cultured and propagated to form hundreds of single-cell-derived clonal populations. Each clonal population was later split into parallel interrogation of its CTP (chance to persist drug treatment), population doubling time, and two transcriptome profiles – 24 hours post Gefitinib drug treatment or mock DMSO treatment. This dataset aimed to find clonally semi-stable molecular signatures that correlate with the observed inherited ability to persist drug treatment.