Pinch loss in early mesenchymal progenitors alters bone marrow environment

To investigate the alteration of bone marrow environment caused by Pinch loss, we performed single-cell RNA-sequencing on bone marrow cells, including hematopoietic cells and non-hematopietic stromal cells. As results indicated, we found both cell type proportion and transcriptomic signatures were dramatically altered by Pinch loss. We identified genes related to ossification, extracellular matrix organization, skeletal system development, and vasculature structure development were significantly differentially expressed between control group and dKO group in many mesenchymal stromal cells. Our results revealed Pinch loss disrupted homeostasis of bone marrow niche, monocytes in bone marrow were actively involved in inflammatory response, and functions of mesenchymal stromal cells was severely disrupted. Overall design: We collected six samples, and two of them were hematopoietic cells from healthy control, two of them were hematopoietic cells from Pinch loss mice, one of them was non-hematopoietic cells from healthy control, one of them was non-hematopoietic cells from Pinch loss mice. In each sample, cells from three mice were pooled together.