Hippocampal RNA-Seq Data and Behavioral Data from an F2 intercross of Rats Selectively-Bred to Show High or Low Levels of Locomotor Activity in a Novel Environment (bred High Responders vs. bred Low Responders)

Using selective breeding, we have produced two rat lines with highly contrasting heritable behaviors: bred Low Responders (bLR) are extremely inhibited and anxious in a novel environment, whereas bred High Responders (bHR) are exploratory and sensation-seeking. These traits map onto temperamental extremes predictive of internalizing vs. externalizing psychiatric disorders in humans and can model two divergent paths to drug abuse. To elucidate gene expression underlying these behavioral extremes, bHRxbLR were bred to produce a F0-F1-F2 cross. We then measured exploratory locomotion, anxiety-like behavior, and sensitivity to reward cues (Pavlovian Conditioned Approach) and performed hippocampal RNA sequencing in both F0s (n=24, n=6/phenotype/sex) and F2s (n=250, n=125/sex). The data included in this GEO upload is the data from the F2s from this experiment.我们通过选择性繁育构建了两个遗传行为差异显著的大鼠品系：选育低反应型（bred Low Responders，bLR）在新环境中表现出极度的抑制与焦虑状态，而选育高反应型（bred High Responders，bHR）则具备探索性与感觉寻求特质。 上述特质对应人类内化型与外化型精神障碍的预测性气质极端类型，同时可模拟药物滥用的两种不同发病路径。 为阐明这些极端行为表型背后的基因表达调控机制，我们通过bHR与bLR杂交构建了F0-F1-F2世代杂交群体。随后分别对F0代（n=24，每种表型/性别各6只）与F2代（n=250，每种性别各125只）大鼠，检测了探索性运动、类焦虑行为以及奖赏线索敏感性（巴甫洛夫条件趋近任务，Pavlovian Conditioned Approach），并对其海马组织开展RNA测序。 本次上传至GEO的数据集即为本实验中F2代大鼠的相关实验数据。