ChIP-Seq of Ndt80-Myc in S. cerevisiae during meiosis and sporulation

The purpose of this experiment was to identify the genes bound by Ndt80 in S. cerevisiae during meiosis and sporulation. Ndt80 was tagged with c-myc and the protein was immunoprecipitated with a c-myc antibody. Cells were grown in liquid YPA (2% Peptone, 2% Potassium Acetate, 1% Yeast Extract) at room temperature for 22 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.本实验旨在鉴定酿酒酵母（Saccharomyces cerevisiae，简称S. cerevisiae）在减数分裂与孢子形成过程中结合Ndt80的基因。将Ndt80进行c-myc标签标记后，采用c-myc抗体对靶蛋白进行免疫沉淀。将细胞置于室温下的液体YPA培养基（含2%蛋白胨、2%乙酸钾、1%酵母提取物）中培养22小时。每组实验重复两次，并通过Illumina HiSeq 4000平台完成测序。