Experiment 1 % coverage.xlsx

To determine how DEX treatment in WT and MKO mice leads to muscle atrophy, we measured muscle protein fractional synthesis rates using shot-gun LC-MS/MS analysis after <i>in vivo</i> ²H₂O labeling . Mice were treated with PBS or DEX for 10 days. During the final 7 days, mice were also labeled with ²H₂O. The sample size for wild type controls treated with PBS (WT-PBS, n=6), wild type treated with DEX (WT-DEX, n=6), MKO treated with PBS (MKO-PBS, n=5) and MKO treated with DEX (MKO-DEX, n=5). The rational for biological replicates of n=5 -6 were used to ensure accurate comparison among each group. Biological replicates were chosen to asses biological variability rather than sole reliance on technical replicates. Controls included WT-PBS treated mice (n=6). Two separate dynamic proteomic experiments were completed and combined for final data analysis. This consisted of experiment 1 that included WT-PBS (n=4) and WT-DEX (n=4). Experiment 2 included the final biological replicates of WT-PBS (n=2), WT-DEX (n=2), MKO-PBS (n=5) and MKO-DEX (n=5). The data sets were combined and annotated as described by the following.<br>Entry name, accession number, group number, sub group number, number of spectra, number of unique peptides, unique score, percent coverage, coverage map, total intensity, protein molecular weight, database, parent charge, score, sequence, chromatographic peak width, parent mass to charge ratio, delta parent mass and delta parent mass in ppm are shown. <br>

为探究野生型（Wild Type, WT）与MKO小鼠经地塞米松（Dexamethasone, DEX）处理后诱发肌肉萎缩的机制，本研究通过体内（in vivo）氘代水（²H₂O）标记结合鸟枪式液相色谱-串联质谱（shot-gun LC-MS/MS）分析，测定了肌肉蛋白质的合成分数速率。
小鼠经磷酸盐缓冲液（Phosphate Buffered Saline, PBS）或DEX处理10天，且在处理的最后7天同步接受²H₂O标记。各组样本量设置如下：经PBS处理的WT对照组（WT-PBS，n=6）、经DEX处理的WT组（WT-DEX，n=6）、经PBS处理的MKO组（MKO-PBS，n=5）以及经DEX处理的MKO组（MKO-DEX，n=5）。本研究采用n=5~6的生物学重复，旨在确保各组间的准确比较；选择生物学重复而非仅依赖技术重复，是为了评估真实的生物学变异。
本研究共开展2次独立的动态蛋白质组学实验，最终合并数据用于分析：实验1包含WT-PBS（n=4）与WT-DEX（n=4）两组样本；实验2包含剩余的生物学重复样本：WT-PBS（n=2）、WT-DEX（n=2）、MKO-PBS（n=5）及MKO-DEX（n=5）。
合并后的数据集将按以下字段完成注释：条目名称、登录号、组号、亚组号、谱图数、唯一肽段数、唯一得分、覆盖率百分比、覆盖图谱、总强度、蛋白质分子量、数据库、母电荷、得分、序列、色谱峰宽、母质荷比、母质量偏差及以ppm为单位的母质量偏差。