Additional file 3 of A proteomics approach to decipher the molecular nature of planarian stem cells

Additional file 3:Comparing the results presented in this manuscript with previously published studies relating to stem cells. Comparison of candidate neoblast protein sequences presented in this paper with genes reported in other proteomic studied to be related to stem cells [25–30] and with specific neoblast-related genes identified in two different high-throughput approaches [32, 33]. From the URFs database, only those sequences with a positive decoy were selected. NCBI BLASTP [31] (min e-value = 0.001) was used on sequence comparison. Sequences were clustered according to their homology and they are listed in the table by their original GI identifier from the corresponding NCBI database. (XLS 816 KB)

附加文件3：本手稿所呈现的结果与既往已发表的干细胞相关研究的对比分析。将本文所呈现的候选新生干细胞（neoblast）蛋白序列，与其他蛋白质组学研究中报道的干细胞相关基因[25–30]，以及通过两种不同高通量手段鉴定得到的特异性新生干细胞相关基因[32, 33]进行比对。从未注释阅读框（URFs）数据库中，仅筛选出带有阳性诱饵的序列。序列比对采用NCBI BLASTP工具[31]，设定最小E值为0.001。研究人员依据序列同源性对其完成聚类，并依照对应NCBI数据库中的原始GI标识符，将所有序列在表格中逐一列出。（XLS格式，816 KB）