Proteins that interact with PHAX and UAP56 from HeLa nuclear extracts
收藏NIAID Data Ecosystem2026-05-02 收录
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HeLa nuclear extracts and RNase A were incubated with GST-PHAX and FLAG-UAP56 in the presence of ATP. The mixture was subjected to immunoprecipitation. The bound proteins were eluted with FLAG peptide. The eluate was subjected to GST pull-down. The bound proteins were treated with HRV 3C protease. Supernatants were analysed by SDS-PAGE and mass spectrometry.
创建时间：
2025-06-28
