Figure S1 - Cloning and Characterization of a Novel Drosophila Stress Induced DNase

Peptide sequence of the CG9989 (SID) protein and comparison with other potential fly nuclease family members. (A) The complete peptide sequence of the CG9989 open reading frame is shown with the critical RGH catalytic residues and the region used to generate anti-SID antibodies are shown in bold and underlined. (B) Alignment of SID with putative Drosophila melanogaster nuclease family members. Sequence alignment was performed with ClustalW (http://www.ebi.ac.uk/Tools/msa/clustalw2/). Shading was performed with Boxshade (http://www.ch.embnet.org/software/BOX_form.html). The protein with the highest identity scores are shown in the order of similarity to SID (e-values on the side). The putative proteins encoded by the CG33346 ORF, CG12917, and CG14062 were found to have a high degree of similarity to SID (see e values next to alignment). Four additional ORFs were found with lower overall similarity but a perfectly conserved RGH motif (red shaded residues) and cofactor binding residue (see red shade) and these are CG14120 (7.8 e-7), CG3819 (4.5 e-6), CG14118 (4.6 e-6), and CG6839 (3.4 e-4). Dashes at the end of the alignment indicate that the protein sequence continues beyond that point. Note that the CG14120 ORF encodes a very large polypeptide that contains two nearly identical nuclease domains that have much lower similarity with the SID protein but both have a conserved RGH motif. (PDF)