Additional file 1 of Metabolomic biomarkers of habitual B vitamin intakes unveil novel differentially methylated positions in the human epigenome

Additional file 1: Table S1. Cohort characteristics for the B vitamin metabolomic biomarker discovery. Table S2. Blood metabolites associated with the dietary intake of folate (B9) and vitamins B6 and B12 (metabolome-wide Bonferroni-adj. p < 0.05). Table S3. B vitamin metabolomic biomarker associations adjusted for diet quality (AHEI-2010) and total energy intake (metabolome-wide Bonferroni-adj. p < 0.05). Table S4. B vitamin metabolomic biomarker associations with other nutrient intakes (metabolome-wide Bonferroni-adj. p < 0.05). Table S5. Summary of nutrients associated with the B vitamin metabolomic biomarkers identified (metabolome-wide Bonferroni-adj. p < 0.05). Table S6. B vitamin metabolomic biomarker associations with circulating blood levels of folate (B9), vitamin B12, hcy and hcy-s. Table S7. Metabolite associations with circulating blood levels of folate (B9), vitamin B12, hcy and hcy-s (metabolome-wide Bonferroni-adj. p < 0.05). Table S8. Cohort characteristics for the B vitamin metabolomic biomarker epigenome-wide meta-analysis. Table S9. Annotation of the epigenome-wide association meta-analysis of folate (B9) and vitamins B6 and B12 biomarker metabolites (epigenome-wide Bonferroni-adj. p < 0.05). Table S10. Epigenome-wide association of folate (B9) and vitamins B6 and B12 biomarker metabolites in the KORA F4 cohort (epigenome-wide Bonferroni-adj. p < 0.05). Table S11. Epigenome-wide association of folate (B9) and vitamins B6 and B12 biomarker metabolites in the TwinsUK cohort (epigenome-wide FDR = 10%). Table S12. Cohort characteristics for the B vitamin intake epigenome-wide meta-analysis. Table S13. DMP results in DMP results in the epigenome-wide association meta-analysis of folate (B9) and vitamin B6 dietary intakes. Table S14. Cis expression quantitative trait methylation signals from the BIOS consortium for the DMP identified in this study. Table S15. Gene expression results for the genes annotated in the biomarker metabolite EWAS meta-analysis.

附加文件1：
表S1：B族维生素代谢组学生物标志物发现研究的队列特征
表S2：与叶酸（B9）、维生素B6及维生素B12膳食摄入相关的血液代谢物（代谢组层面邦费罗尼校正P值<0.05）
表S3：校正饮食质量（美国健康饮食指数2010，Alternative Healthy Eating Index-2010, AHEI-2010）与总能量摄入后的B族维生素代谢组学生物标志物关联分析结果（代谢组层面邦费罗尼校正P值<0.05）
表S4：B族维生素代谢组学生物标志物与其他营养素摄入的关联分析结果（代谢组层面邦费罗尼校正P值<0.05）
表S5：本研究鉴定的B族维生素代谢组学生物标志物相关营养素汇总（代谢组层面邦费罗尼校正P值<0.05）
表S6：B族维生素代谢组学生物标志物与循环血液中叶酸（B9）、维生素B12、同型半胱氨酸（homocysteine, Hcy）及同型半胱氨酸硫内酯（homocysteine thiolactone, Hcy-S）水平的关联分析结果
表S7：与循环血液中叶酸（B9）、维生素B12、Hcy及Hcy-S水平相关的代谢物（代谢组层面邦费罗尼校正P值<0.05）
表S8：B族维生素代谢组学生物标志物表观基因组全关联荟萃分析的队列特征
表S9：叶酸（B9）、维生素B6及维生素B12生物标志物代谢物的表观基因组全关联荟萃分析注释结果（表观基因组层面邦费罗尼校正P值<0.05）
表S10：KORA F4队列中叶酸（B9）、维生素B6及维生素B12生物标志物代谢物的表观基因组全关联分析结果（表观基因组层面邦费罗尼校正P值<0.05）
表S11：英国双胞胎队列（TwinsUK）中叶酸（B9）、维生素B6及维生素B12生物标志物代谢物的表观基因组全关联分析结果（表观基因组层面错误发现率（False Discovery Rate, FDR）=10%）
表S12：B族维生素摄入表观基因组全关联荟萃分析的队列特征
表S13：叶酸（B9）与维生素B6膳食摄入的表观基因组全关联荟萃分析中的差异甲基化位点（Differentially Methylated Position, DMP）分析结果
表S14：本研究鉴定的差异甲基化位点的BIOS联盟（BIOS Consortium）顺式表达数量性状甲基化信号
表S15：本研究生物标志物代谢物表观基因组全关联荟萃分析所注释基因的基因表达结果