A comparison of the sealing abilities between Biodentine and MTA as root-end filling materials and their effects on bone healing in dogs after periradicular surgery

ABSTRACT Objectives: To compare the sealing ability and biocompatibility of Biodentine with mineral trioxide aggregate (MTA) when used as root-end filling materials. Methodology: The Cell Counting Kit-8 (CCK-8) assay was used to compare the cytotoxicity of MTA and Biodentine. Twenty-one extracted teeth with a single canal were immersed in an acidic silver nitrate solution after root-end filling. Then, the volume and depth of silver nitrate that infiltrated the apical portion of the teeth were analyzed using micro-computed tomography (micro-CT). Seventy-two roots from 3 female beagle dogs were randomly distributed into 3 groups and apical surgery was performed. After six months, the volume of the bone defect surrounding these roots was analyzed using micro-CT. Results: Based on the results of the CCK-8 assay, MTA and Biodentine did not show statistically significant differences in cytotoxicity (P>0.05). The volume and the depth of the infiltrated nitrate solution were greater in the MTA group than in the Biodentine group (P<0.05). The volume of the bone defect was larger in the MTA group than in the Biodentine group. However, the difference was not significant (P>0.05). The volumes of the bone defects in the MTA and Biodentine groups were smaller than the group without any filling materials (P<0.05). Conclusions: MTA and Biodentine exhibited comparable cellular biocompatibility. Biodentine showed a superior sealing ability to MTA in root-end filling. Both Biodentine and MTA promoted periradicular bone healing in beagle dog periradicular surgery models.

摘要
研究目的：比较Biodentine与三氧化矿物凝聚体（mineral trioxide aggregate, MTA）用作根尖倒充填材料时的封闭性能与生物相容性。
研究方法：采用细胞计数试剂盒-8（Cell Counting Kit-8, CCK-8）检测法比较MTA与Biodentine的细胞毒性。选取21颗单根管离体牙，行根尖倒充填后将其浸入酸性硝酸银溶液中，随后采用显微计算机断层扫描（micro-computed tomography, micro-CT）分析渗入牙根尖部的硝酸银溶液体积与深度。从3只雌性比格犬获取72个牙根，随机分为3组并施行根尖手术。术后6个月，采用micro-CT分析各组牙根周围骨缺损体积。
研究结果：CCK-8检测结果显示，MTA与Biodentine的细胞毒性无统计学显著差异（P>0.05）。MTA组中渗入的硝酸银溶液体积与深度均大于Biodentine组（P<0.05）。MTA组的骨缺损体积大于Biodentine组，但差异无统计学意义（P>0.05）。MTA组与Biodentine组的骨缺损体积均小于未使用充填材料组（P<0.05）。
研究结论：MTA与Biodentine具有相当的细胞生物相容性。在根尖倒充填中，Biodentine的封闭性能优于MTA。在比格犬根尖周手术模型中，Biodentine与MTA均可促进根尖周骨愈合。