Serological and molecular detection of Leptospira spp in dogs

Abstract INTRODUCTION: This study aimed to detect anti-Leptospira spp antibodies and Leptospira DNA in domestic dogs. METHODS: Blood and urine from 106 dogs were evaluated by microscopic agglutination test (MAT) and polymerase chain reaction (PCR), respectively. RESULTS: Six (5.7%) and one (1%) animals were positive by MAT and PCR, respectively. CONCLUSIONS: These results show a low prevalence of infection by Leptospira spp. The absence of positive results for the Icterohaemorrhagiae serogroup indicates the small relevance of these dogs as sources of human leptospirosis.

摘要与引言：本研究旨在检测家养犬体内的抗钩端螺旋体属（Leptospira spp）抗体及钩端螺旋体DNA。
方法：对106只家养犬的血液与尿液样本，分别采用显微镜凝集试验（microscopic agglutination test, MAT）与聚合酶链式反应（polymerase chain reaction, PCR）进行检测。
结果：经显微镜凝集试验检测呈阳性的受试犬共6只（占比5.7%），经聚合酶链式反应检测呈阳性的共1只（占比1%）。
结论：本研究结果显示，受试犬的钩端螺旋体属感染率较低。未检出黄疸出血群（Icterohaemorrhagiae血清群）阳性样本，表明此类家养犬作为人类钩端螺旋体病传染源的相关性较低。