H3K27me3, H3K79me2, and Suz12 ChIP-Seq in Human Embryonic Stem Cells (BG03).

H3K27me3 is a chromatin modification depositied by Suz12, a component of the Polycomb Group 2 complex, and is associated with transcriptional repression. In contrast, H3K79me2 is a chromatin modification associated with active gene transcription. It is deposited by the histone methyltransferase Dot1L and generally is localized just downstream of the transcriptional start site and extends down the body of the gene. To gain insight into the transcriptional state of genes in hES cells, chromatin immunoprecipitation coupled with massive parallel sequencing (ChIP-seq) was performed to determine the genome-wide occupancy of the H3K27me3 and H3K79me2 chromatin modifications and genome-wide occupancy of the Suz12.

H3K27me3是一种由多梳蛋白复合体2（Polycomb Group 2 complex）的组分Suz12催化沉积的染色质修饰，与转录抑制相关。与之相对，H3K79me2是一类与活跃基因转录相关的染色质修饰，由组蛋白甲基转移酶Dot1L（histone methyltransferase Dot1L）催化沉积，通常定位于转录起始位点下游紧邻区域，并沿基因本体延伸。为深入解析人类胚胎干细胞（hES cells）中基因的转录状态，本研究采用染色质免疫共沉淀结合高通量测序（ChIP-seq）技术，对H3K27me3、H3K79me2两种染色质修饰以及Suz12的全基因组结合占据情况进行了测定。