Rooting of Tineke and Natal Briar rose stem cuttings in different substrates

ABSTRACT: The commercial propagation of rose bushes is carried out asexually by the cutting method. The genetic material and the substrate are factors that interfere in the rhizogenic process of the cuttings. Thus, the aim of this work was to evaluate the effect of the substrate on the rooting of cuttings of two hybrid rose bushes. Floriferous branches of matrix plants were cut at the apex and at the base, approximately 8cm long and with two buds, and the basal leaf was removed. The experimental design was completely randomized, in a 2 × 3 factorial scheme (two cultivars, Tineke and Natal Briar × three substrates, carbonized rice husk, coconut fiber and TN Gold®) containing four replicates of 15 cuttings per replicate. The base of the cuttings was immersed for five seconds in IBA 2000mg L-1 solution (indole butyric acid). The cuttings were placed in trays with 24 cells previously filled with substrate and deposited on the floor in a transparent plastic tunnel, built inside a stove. After the 50-day experiment, rootstocks (%), sprouts (%) and dead (%), root length (cm), fresh and dry shoot biomass and roots (g) were evaluated. The rooting percentage of the Natal Briar cultivar was 75%, thus superior to the Tineke cultivar, which had a rooting percentage of 39%. The best substrates for the Tineke and Natal Briar rose cuttings are CAC and coconut fiber, which provided rooting percentages of 81% and 67% of the cuttings, respectively.

摘要：商业规模化繁殖蔷薇灌木通常采用无性扦插的方式开展。遗传材料与栽培基质是影响插穗生根进程的核心因素。为此，本研究旨在探究栽培基质对两种杂交蔷薇灌木插穗生根的影响。选取母株的开花枝条，于顶端与基部截取长约8cm、带有2个芽点的插穗，并去除基部叶片。本实验采用完全随机设计的2×3析因方案：设置2个蔷薇品种（Tineke与Natal Briar）与3种栽培基质（碳化稻壳、椰糠与TN Gold®），每组设置4次重复，每次重复包含15个插穗。将插穗基部置于2000mg·L⁻¹的吲哚丁酸（indole butyric acid，IBA）溶液中浸泡5秒。随后将插穗放置于预先装填好基质的24孔育苗托盘内，再将托盘置于搭建于恒温箱内的透明塑料拱棚中。实验持续50天后，测定并统计生根插穗率（%）、萌芽率（%）、死亡率（%）、根长（cm）以及地上部与根系的鲜、干生物量（g）。结果表明，Natal Briar品种的插穗生根率达75%，显著高于Tineke品种的39%。Tineke与Natal Briar蔷薇插穗的最优栽培基质分别为CAC与椰糠，二者对应的插穗生根率分别达81%与67%。