Blood gas analyzer utility in evaluating oxygen kinetics of the aqueous humor

Purpose: To measure the partial pressure of oxygen (PO2) and carbon dioxide (PCO2) and the pH of aqueous humor (AH) and arterial blood samples from rabbits using a blood gas analyzer. Methods: Twenty New Zealand rabbits were anesthetized intramuscularly with ketamine and xylazine and were then allowed to breathe room air. Using a gas blood analyzer, arterial blood and AH samples were analyzed for PO2, PCO2, and pH. Results: The mean arterial blood pressure was 87.14 ± 15.0 mmHg. The mean blood and AH PO2 were 95.18 ± 11.76 mmHg and 88.83 ± 9.92 mmHg, the mean blood and AH PCO2 were 25.86 ± 5.46 mmHg and 29.50 ± 5.36 mmHg, and the mean blood and AH pH were 7.38 ± 0.06 and 7.33 ± 0.09, respectively. Conclusion: Conclusions: The blood gas analyzer was easily employed to evaluate the aqueous humor in rabbits. When comparing the results of studies evaluating aqueous PO2, care should be taken to determine the methods used in these studies.

研究目的：使用血气分析仪（blood gas analyzer）测定家兔房水（aqueous humor，AH）与动脉血样本中的氧分压（partial pressure of oxygen，PO2）、二氧化碳分压（carbon dioxide，PCO2）以及pH值。
实验方法：选取20只新西兰家兔，经肌内注射氯胺酮（ketamine）与甲苯噻嗪（xylazine）进行麻醉，随后使其呼吸室内空气。采用血气分析仪对动脉血及房水样本的PO2、PCO2与pH值进行检测分析。
实验结果：家兔的平均动脉压为87.14±15.0 mmHg。动脉血与房水的平均PO2分别为95.18±11.76 mmHg与88.83±9.92 mmHg；平均PCO2分别为25.86±5.46 mmHg与29.50±5.36 mmHg；平均pH值分别为7.38±0.06与7.33±0.09。
研究结论：本研究证实，采用血气分析仪可便捷地对家兔房水进行检测分析。在对比不同研究中房水PO2的检测结果时，应首先明确各研究所采用的实验方法。